Research Article
Aucubin Impeded Preosteoclast Fusion and Enhanced CD31hi EMCNhi Vessel Angiogenesis in Ovariectomized Mice
Figure 6
Aucubin inhibits the MAPK and NF-κB signaling pathways. (a) Protein levels of the MAPK family were analyzed by WBs. (b–d) Quantification of MAPK family protein expression. compared to the Rankl group at 20 min; # compared to the Rankl group at 30 min. (e) Protein levels of the NF-κB family were analyzed by WBs. (f) Quantification of NF-κB family protein level. compared to the Rankl group at 20 min; # compared to the Rankl group at 30 min. (g) Location of p65 fluorescence was observed via IF. Scale bar: 50 μm. (h) Relative p65 fluorescence intensity was analyzed. compared to the control group; # compared to the Rankl group. (i) Relative p65 mRNA was analyzed. compared to the control group; compared to the Rankl group.
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