Rcor2 Is Required for Somatic Differentiation and Represses Germline Cell Fate

Figure 1

Rcor2 is required for mouse embryonic stem cell proliferation. (a, b) The colonies of wild type (a) and Rcor2^-/- (b) mESCs cultured in 2i medium. Scale bars, 100 μm. (c) Genotyping of WT mESCs and Rcor2^-/- mESCs. (d) Western blot of Rcor2 protein level in WT mESCs and Rcor2^-/- mESCs. (e) Relative expression of Rcor2 in WT mESCs and Rcor2^-/- mESCs. (f, g) Relative expression of pluripotent (f) and EMT (g) related genes in WT mESCs and Rcor2^-/- mESCs. (h) Relative cell number of WT mESCs and Rcor2^-/- mESCs after 3 days culture. (i, j) Relative expression of cell cycle-related genes which promote proliferation (i) and inhibit proliferation (j) in WT mESCs and Rcor2^-/- mESCs. Data are expressed as , , , , .

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