Rcor2 Is Required for Somatic Differentiation and Represses Germline Cell Fate

<div><i>Rcor2</i> is required for differentiation of all three germ layers. (a–i) Relative expression of endoderm (a, d, g), mesoderm (b, e, h), and ectoderm (c, f, i) specific genes in WT mESC-derived cells and <i>Rcor2</i><sup><i>-/</i>-</sup> mESC-derived cells at day 2 (a–c), day 4 (d–f), and day 6 (g–i) of spontaneous random differentiation. (j, k) Immunofluorescence staining for DAPI (blue) and <i>α</i>-actinin (red) in WT (j) and <i>Rcor2<sup>-/-</sup></i> (k) mESC-derived cells at day 9 of differentiation. Scale bars, 100 <i>μ</i>m. (l) Relative expression of cardiomyocyte maker genes in WT mESC-derived cells and <i>Rcor2<sup>-/-</sup></i> mESC-derived cells at day 9 of differentiation. 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Stem Cells International

fig2

Figure 2

Figure 2: Rcor2 Is Required for Somatic Differentiation and Represses Germline Cell Fate 