Rcor2 Is Required for Somatic Differentiation and Represses Germline Cell Fate

<div><i>Rcor2</i> knockout promotes PGC differentiation. (a–c) Relative expression of PGC marker genes in WT and <i>Rcor2<sup>-/-</sup></i> mESC-derived cells at day 2 (a), day 4 (b), and day 6 (c) of spontaneous random differentiation. (d, e) mRNA expression level of <i>Dppa3</i> (d) and <i>Dazl</i> (e) during spontaneous random differentiation at day 0, day 2, day 4, and day 6. (f, g) Immunofluorescence staining for DAPI (blue) and Stella (red) in WT (f) and <i>Rcor2<sup>-/-</sup></i> (g) mESC-derived cells at day 9 of spontaneous random differentiation. Scale bars, 100 <i>μ</i>m. (h) Flow cytometric analysis of Stella positive cell in WT and <i>Rcor2<sup>-/-</sup></i> mESC-derived cells during spontaneous random differentiation at day 0, day 2, day 4, and day 6. 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Stem Cells International

fig4

Figure 4

Figure 4: Rcor2 Is Required for Somatic Differentiation and Represses Germline Cell Fate 