Rcor2 Is Required for Somatic Differentiation and Represses Germline Cell Fate

Figure 5

Rcor2 inhibits Dppa3 and Dazl expression by enhancing H3K9me3 on their promoters. (a) The protein level of H3K4me1 and H3K9me3 in WT and Rcor2^-/- mESCs. (b, e) Diagrams of the tested regions by ChIP-qPCR on Dppa3 (b) and Dazl (e) genes and basal H3K9me3 level on Dppa3 (b) and Dazl (e) genes in mESCs (data from GEO: GSM32218, generated by ENCODE [27]). (c, f) ChIP-qPCR shows the enrichment of Rcor2 on Dppa3 (c) and Dazl (f) promoters at the tested regions, relative to their respective negative control regions, which are located in gene bodies (not shown). Data are expressed as , , . (d, g) ChIP-qPCR shows the H3K9me3 level at the tested regions of Dppa3 (d) and Dazl (g) genes in WT and Rcor2^-/- mESCs. Data are expressed as , , , , .

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