Oral Cancer Stem Cell-Derived Small Extracellular Vesicles Promote M2 Macrophage Polarization and Suppress CD4⁺ T-Cell Activity by Transferring UCA1 and Targeting LAMC2

Figure 4

OSCC-CSC-derived sEVs (OSCC-CSC-sEVs) promote M2 polarization of macrophages by transferring UCA1 and targeting LAMC2. (a) UCA1 expression in Cal27-sEVs and Cal27-CSC-sEVs, measured by RT-qPCR. (b) Detection of UCA1 silencing efficiency, determined by RT-qPCR. (c) UCA1 expression in sEVs from Cal27-CSCs treated with sh-UCA1, detected by RT-qPCR. (d) RT-qPCR determination of the expression of the UCA1 and M2 macrophage markers CD163, IL-10, and Arg-1 in macrophages cocultured with sEVs from Cal27-CSCs treated with sh-UCA1. (e) Detection of M2 macrophages after macrophage coculture with sEVs from Cal27-CSCs treated with sh-UCA1, determined by flow cytometry. (f) The expression of M2 macrophage markers (CD163, IL-10, and Arg-1) in macrophages after oe-UCA1 and sh-LAMC2 treatment, determined by RT-qPCR. (g) Detection of CD206⁺CD11b⁺ M2 macrophages after oe-UCA1 and sh-LAMC2 treatment, determined by flow cytometry. (h) The proliferation number of CD4⁺ T cells and the proportion of IFN-γ⁺ T cells after coculture with macrophages treated with oe-UCA1 and sh-LAMC2, detected by flow cytometry. (i) Transwell assay detection of the migration and invasion ability of Cal27 cells cocultured with macrophages treated with oe-UCA1 and sh-LAMC2 (100×). , ^#. All cell experiments were repeated three times.

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