Species-Specific Enhancer Activity of OCT4 in Porcine Pluripotency: The Porcine OCT4 Reporter System Could Monitor Pluripotency in Porcine Embryo Development and Embryonic Stem Cells

Figure 6

Expression of the fluorescent signal in porcine embryonic stem cells using the single-color or dual-reporter system. The porcine OCT4 reporter system consists of two reporters: DE-GFP and PE-RFP. Because each reporter is separate from the other, the reporter system can be used simultaneously. Control: porcine embryonic stem cells without any OCT4 reporter system. GFP reporter: porcine embryonic stem cells with the DE-GFP reporter vector only. RFP reporter: porcine embryonic stem cells with the PE-RFP reporter vector only. GFP/RFP reporter: porcine embryonic stem cells with the dual-OCT4 reporter system.