Asymptomatic Hyperuricemia Is Associated with Achilles Tendon Rupture through Disrupting the Normal Functions of Tendon Stem/Progenitor Cells

Figure 3

Effects of UA on the nontenocytic differentiation of TSPCs. TSPCs were subjected to three types of differentiation media with the addition of PBS or various concentrations of UA for 21 days. (a–c) Subsequent to the differentiation course, oil red O staining (a), alizarin red staining (b), and safranin O staining (c) were performed to assess changes in adipogenesis, osteogenesis, and chondrogenesis of rat TSPCs, respectively. Representative images of each group show morphological alterations and visible changes in staining intensity ( each). . (d–f) Real-time qPCR analysis was applied to determine expression of marker genes in TSPC postcomplete differentiation. PPARγ was used as the marker of adipogenic differentiation (d), with osteopontin for osteogenic (e), and collagen type 2 for chondrogenic differentiation (f). Values are the from three independent experiments. Data were analyzed using two-way ANOVA. ; ; versus PBS-treated control within each group.

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