Exosomes Derived from Runx2-Overexpressing BMSCs Enhance Cartilage Tissue Regeneration and Prevent Osteoarthritis of the Knee in a Rabbit Model

Figure 2

Ad-Runx2 transfection efficiency and exosome characteristics. (a) The green fluorescent distribution of BMSCs by microscope observation. Original magnification,×100. (b) The mRNA expression of Runx2 determined by qRT-PCR. (c, d) The protein expression of Runx2 measured by western blot analysis. (e) Particle size distribution of exosomes measured by nanoparticle tracking analysis (NTA). (f) Morphology of exosomes observed by transmission electron microscopy (TEM). (g) Exosome surface markers proteins (CD81, TSG101, and CD63) measured using western blotting. (h) Representative immunofluorescence photomicrograph of PKH-26- (green) labelled exosomes absorbed by chondrocytes, and the nuclei of which were stained by DAPI (blue). compared with MSCs, respectively, with the unpaired Student’s -test conducted. The statistical data were measurement data and expressed by of 3 independent experiments. The experiment was performed in triplicates.

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