Exosomes Derived from Runx2-Overexpressing BMSCs Enhance Cartilage Tissue Regeneration and Prevent Osteoarthritis of the Knee in a Rabbit Model

Figure 4

Responses of rbACs stimulated by BMSCs-Exos and R-BMSCs-Exos in 10 μg exosome concentrations/mL. The E-10 indicates the BMSCs-Exos; the RE-10 indicates the R-BMSCs-Exos. (a) Gene expression changes of aggrecan, COL-II, and SOX9 after stimulation by E-10 and RE-10. (b) The OD value of rbACs measured by CCK-8 assay. (c, d) Protein expression levels of aggrecan, COL-II, and SOX9 were detected by western blotting after stimulation by E-10 and RE-10, and the results of statistical analysis also shown. (e) The number of invaded rbACs measured by transwell assay. Original magnification, ×200. (f) Quantitative analysis of the number of cell invasion. (g) Proliferation of rbACs determined by EdU labeling assay. Original magnification, ×200. (h) Quantitative analysis for EdU-positive cells. This experiment was repeated three times. compared to 0; compared to 0; ^& compared to E-10; ^&& compared to E-10.

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