Exosomes Derived from Runx2-Overexpressing BMSCs Enhance Cartilage Tissue Regeneration and Prevent Osteoarthritis of the Knee in a Rabbit Model

Figure 5

Filtering and function of YAP-siRNA. (a, b) YAP-siRNA protein expression levels of filter, action concentration, and action time were detected by western blotting and the results of statistical analysis. compared to NC or 0; compared to NC or 0. (c) The expression of filter, action concentration, and action time determined by qRT-PCR. compared to NC or 0; compared to 0. (d) Gene expression changes of YAP and its target genes (ANKRD1, CTGF, and Cyr61) were detected by qRT-PCR. ; compared to 0; ^& compared to E-10. (e, f) Protein expression levels of YAP and target genes including ANKRD1, CTGF, and Cyr61 were detected by western blotting, and the results of statistical analysis also shown. compared to 0; compared to 0. (g, h)Gene expression changes of YAP and its target genes (ANKRD1, CTGF, and Cyr61) were detected by immunohistochemical staining compared to 0, ^&& compared to E-10. This experiment was repeated three times.

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