Human Umbilical Cord Mesenchymal Stem Cells Encapsulated with Pluronic F-127 Enhance the Regeneration and Angiogenesis of Thin Endometrium in Rat via Local IL-1β Stimulation

Figure 5

PF-127-encapsulated hUC-MSCs facilitate the angiogenesis of the damaged rat uterine. (a) IHC staining of angiogenesis markers (VWF and VEGFA).  μm. (b) Quantitation of VEGFA-positive staining vessels. Data was reported as . Student’s -test was applied for significant difference ( and ns = nonsignificant), . (c) Quantitation of VWF-positive staining vessels. Data was reported as . Student’s -test was applied for significant difference ( and ns = nonsignificant), . (d, e) Q-PCR analyzed the relatively gene expression of angiogenesis markers (Vegfa and Nos3). The expression values were normalized to 18S. Subsequently, the mRNA expression values were calculated as relative mRNA level versus mRNA expression values with saline injection, which was set to 1. Data was reported as . Student’s -test was applied for significant difference ( and ns = nonsignificant), .

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