Research Article
Scrapie-Responsive Gene 1 Promotes Chondrogenic Differentiation of Umbilical Cord Mesenchymal Stem Cells via Wnt5a
Figure 4
SCRG1 overexpression promoted chondrogenic differentiation of UCMSCs. (a–c) mRNA and protein levels of SCRG1 were detected by qRT-PCR and IHC. Results showed that mRNA and protein levels of SCRG1 increased after overexpression of SCRG1. . (d) SOX9, COL2A1, and ACAN mRNA levels increased after overexpression of SCRG1. (e) Alcian Blue staining showed an increase in glycosaminoglycans at day 21 after overexpression of SCRG1. . (f, g) Protein levels of COL2A1 and ACAN detected by IHC at day 21 increased after SCRG1 knockdown. . Experiments were repeated three times; (-test).
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