Scrapie-Responsive Gene 1 Promotes Chondrogenic Differentiation of Umbilical Cord Mesenchymal Stem Cells via Wnt5a

Figure 6

UCMSC^OE-SCRG1 promoted cartilage defects repair in vivo. (a) Schematic of cartilage defect model and UCMSC administration. (b) Photographs of cartilage defects at 12 weeks after UCMSC administration. Obvious defects were observed in the blank group, whereas the surface was smooth and regenerated tissue was tightly connected to surrounding cartilage in the OE-SCRG1 group. (c) H&E, Alcian Blue, and Safranin O/Fast Green staining at 12 weeks after surgery. Results showed that defects in the blank group contained necrotic and fibrous tissue, while the OE-SCRG1 group showed considerable chondrocyte regeneration and tissue morphologies similar to normal surrounding cartilage. (d) ICRS macroscopic scores at 12 weeks after surgery. Scores in the OE-SCRG1group were significantly higher than those in the OE-nc group (). (e) Modified ICRS histological scores at 12 weeks after surgery. Scores were significantly higher in the OE-SCRG1 group than in the OE-nc group (). . ; (-test).

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