miR-3960 from Mesenchymal Stem Cell-Derived Extracellular Vesicles Inactivates SDC1/Wnt/β-Catenin Axis to Relieve Chondrocyte Injury in Osteoarthritis by Targeting PHLDA2

Figure 4

miR-3960 attenuates chondrocyte injury by inhibiting PHLDA2. Chondrocytes were transduced with miR-3960 mimic and/or PHLDA2A. (a) miR-3960 expression and PHLDA2 mRNA level in the chondrocytes determined by RT-qPCR. Chondrocytes were treated with IL-1β and transduced with miR-3960 mimic and/or PHLDA2A. (b) Cell proliferation assessed by CCK8 assay. (c) Apoptosis assessed by flow cytometry. (d) Protein levels of collagen II, aggrecan, MMP13, and ADAMTS5 in the chondrocytes measured by immunoblotting. (e) Levels of IL-6 and TNF-α in the chondrocytes measured by ELISA. vs. chondrocytes treated with mimic-NC+vector or chondrocytes without treatment; ^# vs. chondrocytes treated with mimic-NC+PHLDA2 or chondrocytes treated with IL-1β+mimic-NC+vector; & vs. chondrocytes treated with IL-1β+mimic-NC+PHLDA2. Data are shown as the of three technical replicates. Data between two groups were compared by independent -test. Data comparisons among multiple groups were analyzed by the one-way ANOVA with Tukey’s post hoc test, and comparison of data at different time points was analyzed using repeated measurement ANOVA with Tukey’s post hoc test.

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