miR-3960 from Mesenchymal Stem Cell-Derived Extracellular Vesicles Inactivates SDC1/Wnt/β-Catenin Axis to Relieve Chondrocyte Injury in Osteoarthritis by Targeting PHLDA2

Figure 6

SDC1 induces chondrocyte injury by activating Wnt/β-catenin pathway. (a) β-catenin mRNA level in the cartilage tissues of OA patients () and patients with traumatic amputation () determined by RT-qPCR. Chondrocytes were treated with IL-1β, sh-SDC1, and/or LiCl. (b) mRNA levels of SDC1 and β-catenin in the chondrocytes determined by RT-qPCR. (c) Protein levels of SDC1 and β-catenin in the chondrocytes determined by immunoblotting. (d) Cell proliferation assessed by CCK8 assay. (e) Apoptosis assessed by flow cytometry. (f) Protein levels of collagen II, aggrecan, MMP13, and ADAMTS5 in the chondrocytes measured by immunoblotting. (g) Levels of IL-6 and TNF-α in the chondrocytes measured by ELISA. vs. patients with traumatic amputation or chondrocytes without treatment; ^# vs. chondrocytes treated with IL-1β+control shRNA; & vs. chondrocytes treated with IL-1β+sh-SDC1+PBS. Data are shown as the of three technical replicates. Data between two groups were compared by independent t-test. Data comparisons among multiple groups were analyzed by the one-way ANOVA with Tukey’s post hoc test, and comparison of data at different time points was analyzed using repeated measurement ANOVA with Tukey’s post hoc test.

(a)

(b)

(c)

(d)

(e)

(f)

(g)