Single-Cell RNA Sequencing Reveals the Interaction of Injected ADSCs with Lung-Originated Cells in Mouse Pulmonary Fibrosis

Figure 1

Intratracheal injection of ADSCs alleviated mouse pulmonary fibrosis. (a) The diagram of the study. (b) Kaplan-Meier survival curve for pulmonary fibrosis mice. The group was received PBS (, black line), the BLM + PBS group administered 3.5 mg/kg BLM in 50 ul PBS at day 0 (, red line), and BLM + ADSCs group received 5 × 10^5 cell in 50 ul PBS by intratracheally at day 3 post-BLM administration. (c) Masson’s and H&E staining images of the lung tissues of mice on day 14, scale bar: 200 μm. (d) Density of pulmonary fibrotic tissue () calculated on histological sections of Masson’s trichrome staining. (e) Density of the pulmonary tissue () calculated on histological sections of H&E trichrome staining. (f). Relative expression of TNF-α, IL-1β, IL-10, and IL-6 mRNA in the pulmonary tissue of BLM + ADSC and BLM + PBS group ( each) determined by quantitative real-time polymerase chain reaction, on days 7 and 14. Data presented as with significance based on Student -test. ; .

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