Single-Cell RNA Sequencing Reveals the Interaction of Injected ADSCs with Lung-Originated Cells in Mouse Pulmonary Fibrosis

Figure 4

Recollected (ADSC_rec) ADSCs showed a different single-cell transcriptomic profile compared to cultured ADSCs (ADSC_cul). (a) UMAP distribution of in vitro cultured (ADSC_cul) and recollected (ADSC_rec) ADSCs merged into one dataset. (b) UMAP distribution of all 10 clusters identified by Seurat in merged dataset of ADSC_cul and ADSC_rec. (c) Cell count of every cluster in two ADSC groups. (d) Violin plots of differentially expressed genes between ADSC_cul and ADSC_rec groups. (e, f) REACTOME pathway enrichment terms of differentially expressed genes ((e) upregulated genes, (f) downregulated genes) in ADSC_rec group compared to ADSC_cul group. Genes were first filtered by a condition of log2 fold change >0.5 or < -0.5 and adjusted value <0.05.

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