MSC-Derived Extracellular Vesicle-Delivered L-PGDS Inhibit Gastric Cancer Progression by Suppressing Cancer Cell Stemness and STAT3 Phosphorylation

Figure 5

EVs-L-PGDS inhibited the growth of subcutaneous tumors in nude mice induced by gastric cancer cell SGC-7901. (a) Representative images of tumor-bearing mice. (b) Representative view of subcutaneous tumors. (c, d) Weight statistics and volume changes of tumor in mice treated with different EVs and PBS. (e) Western blot for the expression of L-PGDS, Bcl2, and Bax in tumor tissues. (f) Quantitative analyses of protein expression of tumor tissue. (g) HE staining of tumor tissues (×100). Arrowheads point to blood vessels. (h) Immunofluorescence staining for the expression of L-PGDS in tumor tissues (×100). (i) TUNEL assay for the apoptosis level in tumor tissues (×400). (j) Quantitative analyses of TUNEL-positive cells in tumor tissue. (k) Immunohistochemical staining for the expression of PCNA in tumor tissues (×400). (l) Quantitative analyses of PCNA expression in tumor tissue. ; , , and .