Erythropoietin Activates Autophagy to Regulate Apoptosis and Angiogenesis of Periodontal Ligament Stem Cells via the Akt/ERK1/2/BAD Signaling Pathway under Inflammatory Microenvironment

Figure 6

EPO regulated autophagy, apoptosis, and angiogenesis of PDLSCs through the Akt/ERK1-2/BAD signaling pathway under inflammatory microenvironment. (a) Bubble map of KEGG enrichment of RNA sequencing. (b, c) Heat map of enriched genes and qPCR validation (CSF3, ITGA10, PCK2, THBS4, DUSP4, ERGE, and KDR). (d) Protein expression levels of Akt, p-Akt, Erk1/2, p-Erk1/2, BAD, and p-BAD through western blot. (e, f) mRNA and protein expression levels of VEGF-a, FGF-2, IGF-1, Bax/Bcl2, Beclin1, and LC3B through qPCR and western blot. (g) Tube formation under different treatments. (h) Cell apoptosis rate under different treatments. (i, j) Immunofluorescence on DAPI, cytoskeleton, and VEGF-a/LC3B. Data are presented as (); , , , and .

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