In Vitro Characterization of Motor Neurons and Purkinje Cells Differentiated from Induced Pluripotent Stem Cells Generated from Patients with Autosomal Recessive Spastic Ataxia of Charlevoix-Saguenay

<div>Immunofluorescence assessment of sacsin and DRP1 expression in Purkinje cells differentiated from healthy or ARSACS iPSCs cultured in 3D. iPSCs were seeded at a density of 285,000 cells/cm<sup>2</sup> onto the sponge containing fibroblasts for a total in vitro differentiation of 53 days. The 3D model was imaged with a view from above after staining of cells for NFM in green (a, e, d, h), DRP1 in red (b, f, d, h), and sacsin in white (c, g, d, h). Nuclei were stained in blue with DAPI (d, h). Scale bar in (h): 20 <i>μ</i>m.</div>

Stem Cells International

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Figure 3: In Vitro Characterization of Motor Neurons and Purkinje Cells Differentiated from Induced Pluripotent Stem Cells Generated from Patients with Autosomal Recessive Spastic Ataxia of Charlevoix-Saguenay 

Figure 3 | In Vitro Characterization of Motor Neurons and Purkinje Cells Differentiated from Induced Pluripotent Stem Cells Generated from Patients with Autosomal Recessive Spastic Ataxia of Charlevoix-Saguenay 