Review Article

Promising Markers in the Context of Mesenchymal Stem/Stromal Cells Subpopulations with Unique Properties

Table 2

Properties of subpopulations separated from MSC tissues.

MarkerSorting methodEffectReference

CD49fFACS↑ Clonogenity,
↑ proliferation,
↑ migration,
↑ multilineage differentiation,
[26]

CD49fFACS↑ Colony forming,
↑ adipogenic and osteogenic differentiation
[25]

CD49fFASC↑ Self-renewal,
↑ spheres formation,
↑ adipogenic and osteogenic differentiation
[27]

CD49fMASC↑ Adhesion,
↑ proliferation,
↑ adipogenic and osteogenic differentiation,
↑ migration,
↑ antiapoptotic potential
[28]

CD271FACS↑ Clonogenicity,
↑ osteogenic differentiation
[30]

CD271FACS↑ Proliferation,[31]

CD271FACS↑ Proliferation,
↓ osteogenic and adipose differentiation
[47]

CD271MACS↑ Neuronal and glial differentiation,
↑ migration toward islets and islet-like cell clusters
[32]

CD271MACS↑ Pluripotent genes expression[48]

CD271MACSNo changes in multipotent differentiation in vitro,
↑ osteochondral repair in vivo,
↓ angiogenesis in vitro and in vivo
[49]

CD271MACS↑ Adipogenesis, chondrogenesis, osteogenesis[33]

CD349FACS↓ Angiogenic-properties,
↓ vasculogenesis,
↓ re-endothelialization,
similar osteogenic differentiation potential
[35]

CD349FASC↑ Clonogenicity,
↑ multi-lineage differentiation
[36]

GD2FACS↑ Clonogenicity,
↑ proliferation,
↑ adipogenesis,
↑ osteogenesis,
↑ gene expression: LPL, adipsin, collagen I, CBFA1, OC,
↑ content of positive cells: Sca-1+, CD105+, SSEA-1+, Nanog+,
↓ content of positive cells: CD34+, C-kit+, CD45+, CD11b+
[40]

GD2MACS↑ Clonogenicity,
↑ colony size,
↑ proliferation,
↑ gene expression: SSEA-4, Oct-4, Sox-2, Nanog, Nestin, GFAP, NSE,
↑ adipogenesis,
↑ osteogenesis
[37]

Sca-1FACS↑ Clonogenicity,
↑ gene expression: NANOG, TERT, BMP2, Myf5,
↓ chondrogenesis,
↓ gene expression: Col2a1
[41]

Sca-1FACS↑ Clonogenicity[50]

Sca-1FCAS↑ Gene expression: Eng (CD105),
↓ gene expression: IL-6, Pdgfra, Ly6a, Itgb1, Itga5, CD44, Thy1(CD90)
[51]

SSEA-3FACS↑ Differentiation into insulin-producing cells,
↑ pluripotent genes expression
[43]

SSEA-3FACS↑ Migration toward injured liver cells,
↑ restoration of liver function in liver fibrosis model,
in vivo spontaneous differentiation into hepatocyte
[52]

SSEA-3FACS↑ Sphere formation[22]

SSEA-3FACS↑ Sphere formation,
↑ pluripotency,
↑ spontaneous expression and expression after differentiation of markers from three germ layers
[21]

SSEA-3FACSBetter biodistribution,
↑ motor and cognitive functions of HIE rats
[45]

SSEA-3FACS, MACSSimilar effects observed in both sorting methods
↑ sphere formation,
↑ pluripotency,
↑ neural differentiation
[42]

SSEA-3MACS↓ Apoptosis and senescence after UV or H2O2 treatment,
↑ activation of damage repair system of DNA via non-homologous end joining
[53]

SSEA-3MACS↑ Pluripotent genes expression[48]

SSEA-3MACS↑ Expression and secretion of growth factors,
↑ pluripotency gene expression,
↑ increased wound healing of skin ulcers
[44]

SSEA-4FACSNo differences in proliferation, pluripotency genes expression, osteogenic differentiation, adipogenic differentiation[34]

SSEA-4FACS↑ Clonogenicity,
↑ proliferation
[54]

SSEA-4FACS↑ Pluripotency and neural gene expression,
↑ viability of spheres,
smaller spheres formed,
no differences in proliferation and clonogenicity
In submission

SSEA-4MACS↑ Adipogenesis[33]

SSEA-4 CD271FACS↑ Proliferation[31]

SSEA-4 CD271FACS↑ Clonogenicity,
↓ adipogenesis
[55]

Note: FACS: fluorescence-activated cell sorting, MACS: magnetic activated cell sorting, HIE: hypoxic-ischemic encephalopathy.