Bone Marrow-Derived Mesenchymal Stem Cells and γ-Secretase Inhibitor Treatments Suppress Amyloid-β25–35-Induced Cognitive Impairment in Rat Dams and Cortical Degeneration in Offspring

Figure 1

Design of the study: adult female albino rats were subjected to intracerebroventricular (i.c.v.) injection with Aβ25–35 and vehicle 0.9% saline before pregnancy and allowed to recover for 10 days and then subjected to behavioral testes (novel object recognition test and Y-maze test). After that, female rats were mated with males, and pregnant female rats subjected to Aβ25–35 injection were classified into the AD group, AD group that received intravenous injection of MSCs (one million/rat/week), and AD group orally treated with GSI-953 (2.5 mg/kg·b·wt). The neonates were sacrificed at different postnatal days (7, 14, and 21), and the brain cortex was subjected to histological, immunohistochemistry, qRT-PCR, and western blotting studies.