Research Article
Unveiling Cellular Traits of Osteosarcoma Cancer Stem Cells via Bulk and Single-Cell RNA-Sequencing Analysis
Figure 2
(a) Clustering was based on the top 25% variance of the genes in GSE33382. The red color represents the patients’ gender, OS histological subtype, tumor, grade, and metastasis location. Color is related to the different classifications of each clinical phenotype. (b) Determination of the soft-thresholding power (β) with the cutoff at 0.9 in WGCNA and mean connectivity for various soft-thresholding powers. These two figures show that β = 3 may output a high mean number of connections and a scale-free network. (c) Analysis of the scale-free topology model fitting index, R2 = 0.96 means the scale-free model is ideal.
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