Establishment of Epithelial Inflammatory Injury Model Using Intestinal Organoid Cultures

Table 1

Epithelial tight junction changes in intestinal inflammation.

Tight junction		Function	Expression in TNF-α-10 treatment	DSS-induced colitis	TNBS-induced colitis	Expression in CD	Expression in UC
mRNA	Ocln	Translated into Ocln protein	↓	↓ [43]	↓ [40]	↓ [41]	↓ [41]
	Cldn1	Translated into Cldn1 protein	↔	↔ [43]	↔ [40]	↓ [41]; Active^1 ↔ [42]; Inactive^2↔ [42]	↓ [41]; Active ↔ [42]; Inactive ↔ [42]
Protein	Ocln	Binds ZO-1, regulates paracellular permeability, function in cellular adhesion [44]	↓	↓ [43]; ↓ [45]	↓ [46]	↓ [47]; ↓ [41]	↓ [41]
	Cldn1	Tightens the epithelium able to initiate formation of TJ trends [44]	↓	↓ [43]; ↑ [45]	↓ [46]	↓ [41]; Active ↔ [47]	↓ [41]

¹Active represents the sample from the active stage of disease; ²inactive represents the sample from the inactive stage of disease. ↓: decrease; ↑: increase; ↔: no change; CD: Crohn’s disease; UC: ulcerative colitis; Ocln: occludin; Cldn1: claudin-1.