MAGEB2-Mediated Degradation of EGR1 Regulates the Proliferation and Apoptosis of Human Spermatogonial Stem Cell Lines

Figure 2

Expression of MAGEB2 in OA patients with normal spermatogenesis. (a) Representative images of HE staining for testicular tissue from patients with OA. Scale bar, 200 μm. (b) Western blot assay to verify the expression of MAGEB2 in three OA patients. (c) Two-color immunofluorescence staining of MAGEB2-expressing cells. MAGEB2 signals are shown in green, signals of different cellular markers including DDX4, GFRA1, KIT, and PCNA are shown in red, and DAPI-labeled nuclei are shown in blue. Scale bar, 20 μm. (d) Dot plots demonstrated the coexpression abundance of MAGEB2 with DDX4, GFRA1, KIT, and PCNA. The -axis is the proportion of double positive cells, each circle represents the result of counting a circular cross section of a seminiferous tubule, and at least 20 seminiferous tubules were counted.

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