MAGEB2-Mediated Degradation of EGR1 Regulates the Proliferation and Apoptosis of Human Spermatogonial Stem Cell Lines

Figure 3

MAGEB2 overexpression inhibited the proliferation of SSC lines. (a) Western blot assay to verify the overexpression of MAGEB2. (b) The bar graph showed the relative levels of MAGEB2 after overexpression in the SSC lines. (c) The CCK8 assay detected cell proliferation on days 1-5 of MAGEB2 overexpression, with a significant decrease in cell proliferation from day 3. (d) EdU assay examined cell DNA synthesis after MAGEB2 overexpression. (e) The bar graph demonstrates the relative changes in cellular DNA synthesis following MAGEB2 overexpression. Scale bar, 50 μm. (f) Western blot experiments showed expression changes of proteins related to SSC proliferation, such as PLZF, CCND1, and PCNA. (g) The bar graph showed the relative levels of PLZF, CCND1, and PCNA after MAGEB2 overexpression in the SSC lines. OD: optical density; A450: absorbance at 450 nm. represents .

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