MAGEB2-Mediated Degradation of EGR1 Regulates the Proliferation and Apoptosis of Human Spermatogonial Stem Cell Lines

Figure 5

Validation of MAGEB2 candidate target proteins in SSC lines. (a) Prediction of MAGEB2 candidate target proteins using HitPredict, STRING, and GeneMANIA databases. Venn diagram showing the intersection of the predictions from the 3 databases. (b) The violin plot demonstrates the distribution of MAGEB2 and the candidate target proteins EGR1 and HDAC1 in testicular cells at the mRNA level. Each point in the plot represents a cell sample, the -axis represents the expression intensity of the mRNA, and the -axis denotes the probability density of the gene in the cell population. (c) Western blot analysis of EGR1 and HDAC1 expression at the protein level after overexpression of MAGEB2. (d) The bar graph shows the expression levels of EGR1 and HDAC1 after MGEB2 upregulation. (e) Protein immunoprecipitation assay verified the interaction of MAGEB2 with EGR1. (f) Results and models of molecular docking. Rigid protein–protein docking (ZDOCK) was performed between MAGEB2 and EGR1 to study the relationships. The amino acid sites of protein molecule interactions are shown in the table and the ball-and-stick diagram, such as TYR14-GLN153 and ASN16-TYR142. TYR14 represents tyrosine at position 14 of the peptide chain, and the others are similar. indicates . L: ligand; R: receptor; TYR: tyrosine; GLN: glutamine; ASN: asparagine; GLY: glycine; THR: threonine; PRO: proline.

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