MAGEB2-Mediated Degradation of EGR1 Regulates the Proliferation and Apoptosis of Human Spermatogonial Stem Cell Lines

Figure 6

Rescue effects of EGR1 on the proliferation of MAGEB2-overexpressed SSC lines. (a) Western blot detects the protein levels of PLZF, EGR1, MAGEB2, and PCNA after overexpression of MAGEB2, EGR1, or both. (b) The bar graph displays the levels of PLZF, EGR1, MAGEB2, and PCNA after transfection. (c) CCK8 assay detects the proliferation of SSC lines transfected with expression plasmids of MAGEB2, EGR1, or both. (d) EdU assay examines cellular DNA synthesis after transfection. Scale bar, 50 μm. (e) The bar graph shows the percentage of EdU positive cells. (f) Annexin V staining combined with FACS detects apoptosis of SSC lines. (g) The bar plot demonstrates the percentage of early, late, and total apoptotic cells. indicates .

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