Research Article

Combination Therapy with Platelet-Rich Plasma and Epidermal Neural Crest Stem Cells Increases Treatment Efficacy in Vascular Dementia

Figure 2

The rat whiskers pad (a). The isolated individual hair follicles (b). Seven days after explanation, the stem cells began to migrate from the hair follicle (c). Immunostaining was performed against nestin and Sox10 as neural crest stem cell markers. The actin markers with Alexa Fluor 488 conjugated secondary antibodies emitted green fluorescence, scale bar = 50 μm (d, e) The viability of stem cells was also assessed by fluorescein diacetate (FDA) staining, scale bar = 100 μm (f). The cell nuclei were counterstained blue with 4ʹ,6-diamidino-2-phenylindole (DAPI).
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