AT7867 Inhibits the Growth of Colorectal Cancer Stem-Like Cells and Stemness by Regulating the Stem Cell Maintenance Factor Ascl2 and Akt Signaling

<div>Sphere cells derived from HCT116 and HT29 cell lines display CSC characteristics. (a) HCT116 and HT29 were cultured in serum-free medium for 14 days to obtain spheres. (b) The SFE of sphere cells from the first to fifth passage. (c, d) The expression of Oct4, Nanog, CD133, and CD44 by performing the Western blot in total protein. (e, f) The ratio of Aldh+ cells was analyzed by flow cytometry assay. (g, h) Transwell assay in fifth-passage sphere cells and the parental cells; the histogram shows the number of migrated cells per field. (i) Representative pictures showing the tumorigenic capacity of 10<sup>4</sup> fifth-passage CRC sphere cells and 10<sup>4</sup> parental cells. 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Stem Cells International

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Figure 1

Figure 1: AT7867 Inhibits the Growth of Colorectal Cancer Stem-Like Cells and Stemness by Regulating the Stem Cell Maintenance Factor Ascl2 and Akt Signaling 

Figure 1 | AT7867 Inhibits the Growth of Colorectal Cancer Stem-Like Cells and Stemness by Regulating the Stem Cell Maintenance Factor Ascl2 and Akt Signaling 