Research Article
Extracellular Vesicles Secreted by TGF-β1-Treated Mesenchymal Stem Cells Promote Fracture Healing by SCD1-Regulated Transference of LRP5
Figure 8
EV-SCD1 promotes HUVEC proliferation, angiogenesis, and migration by interactions involving LRP5. (a) Expression levels of HIF-1α, VEGF, TAZ, YAP, and p-YAP in HUVECs after transfection with MSCPBS-EVs, MSCTGF-β1-EVs, MSCTGF-β1-EV-si-NC, or MSCTGF-β1-EV-si-SCD1, . (b) Expression level of LRP5 and LRP6 in HUVECs after transfection with MSCPBS-EVs, MSCTGF-β1-EVs, MSCTGF-β1-EV-si-NC, or MSCTGF-β1-EV-si-SCD1, . (c–f) CCK-8 (c), EdU (d), scratch wound assays (e), and tube formation (f) were used to verify the functional role of LRP5 on cell proliferation, migration, and angiogenesis in HUVECs, . Data are expressed as ; , , and . ns: no significance; MSCs: mesenchymal stem cells; EVs: extracellular vesicles; HUVECs: human umbilical vein endothelial cells.
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