Vascularization of a Bone Organoid Using Dental Pulp Stem Cells

Figure 6

Immunofluorescence staining of BMSC/DPSC constructs after endothelial differentiation. (a) CD31 expression in BMSC/DPSC constructs at 5, 10, and 20 days. (b) Photomicrographs of CD31-positive cells (red) and GFP-labelled DPSCs (green) in 80B/20D constructs after 20 days of endothelial differentiation. White arrows indicate the colocalization of CD31 and GFP-transduced DPSCs. Dotted lines indicate the outline of cell constructs. Scale bars: 50 μm.

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