Matricellular Protein SMOC2 Potentiates BMP9-Induced Osteogenic Differentiation in Mesenchymal Stem Cells through the Enhancement of FAK/PI3K/AKT Signaling

Figure 2

The effect of SMOC2 on BMP9-induced early osteogenic marker levels. (a) Real-time PCR assay results showed the effect of SMOC2 on the mRNA expression of RUNX2 induced by BMP9. (b) Western blot results showed the effect of SMOC2 on the protein expression of RUNX2 induced by BMP9 at 24 h and 48 h. (c) The quantification results of the Western blot results showed the effect of SMOC2 on the protein expression of RUNX2 induced by BMP9. (d) Real-time PCR assay results showed the effect of SMOC2 knockdown on the mRNA expression of RUNX2 induced by BMP9. (e–f) Western blot assay and quantification results showed the effect of SMOC2 knockdown on the protein expression of RUNX2 induced by BMP9 at 24 h and 48 h. (g–h) ALP assay and quantification results showed the effect of overexpression or knockdown of SMOC2 on the activity of ALP induced by BMP9 in MEFs. (k) Immunofluorescence staining assay results showed the effect of SMOC2 on the expression of RUNX2 induced by BMP9. Compared with the control group, ; ; compared with the AdBMP9 group, #, ##.

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