Characterization of Primary Cilia Formation in Human ESC-Derived Retinal Organoids

Figure 6

Ciliated AC3-positive RGCs are identified in human retina. (a) Representative images illustrating 68-year-old human retina immunostained with the neuronal ciliary marker, AC3 (gray), Arl13b and gamma-tubulin cilia marker (both in green). RGCs are marked by RBPMS (red), and nuclei are stained with DAPI (blue). White arrows indicate primary cilia. (b) Quantification of the population percentages of 4 subtypes of RGCs defined by ciliary markers. (c) Quantification of the populations of 4 subtypes of RGCs shows that AC3-positive ciliated RGCs are the most abundant (One-way ANOVA). Scale bar: 10 μm; magnified, 1 μm.

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