Extracellular Vesicle-Loaded Oncogenic lncRNA NEAT1 from Adipose-Derived Mesenchymal Stem Cells Confers Gemcitabine Resistance in Pancreatic Cancer via miR-491-5p/Snail/SOCS3 Axis

Figure 2

NEAT1 is delivered into PCa cells via ADSC-EVs. (a) Internalization of PKH67-labeled EVs by SW1990 cells under the fluorescence microscope (scale bar: 25 μm). (b) NEAT1 expression in SW1990 cells cocultured with ADSC-EVs determined by RT-qPCR. (c) Delivery of NEAT1 by SW1990 cells cocultured with GW4869-treated ADSCs with Cy3-labeled NEAT1 overexpression (scale bar: 25 μm). (d) NEAT1 expression in RNase A-treated ADSC-CM determined by RT-qPCR. (e) NEAT1 expression in SW1990 cells cocultured with actinomycin D determined by RT-qPCR. vs. SW1990 cells treated with PBS, ADSCs, or SW1990 cells cocultured with EVs+DMSO. All data were presented as deviation. Comparisons of data between two groups were analyzed by unpaired -test, and comparison of data among multiple groups was tested by one-way analysis of variance, followed by Tukey’s post hoc test. Cell experiments were independently repeated three times.

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