Extracellular Vesicle-Loaded Oncogenic lncRNA NEAT1 from Adipose-Derived Mesenchymal Stem Cells Confers Gemcitabine Resistance in Pancreatic Cancer via miR-491-5p/Snail/SOCS3 Axis

Figure 6

miR-491-5p regulates Snail/SOCS3 in PCa cells. (a) The binding site between miR-491-5p and Snail predicted by StarBase database. (b) The target relationship between miR-491-5p and Snail verified by dual-luciferase reporter gene assay. (c) Snail expression in SW1990 cells in response to miR-491-5p mimic alone or combined with Snail overexpression determined by Western blot analysis. (d) SOCS3 expression in the PCa-related microarray dataset GSE32676 (normal pancreatic tissues: ; PCa tissues: ). (e) SOCS3 mRNA level in SW1990 cells in response to miR-491-5p mimic alone or combined with Snail overexpression determined by RT-qPCR. (f) SOCS3 protein level in SW1990 cells determined by Western blot analysis. SW1990 cells were treated with EVs. (g) Protein levels of Snail and SOCS3 in SW1990 cells in response to EVs determined by Western blot analysis. (h) Protein levels of Snail and SOCS3 in SW1990 cells in response to overexpression of NEAT1 alone or combined with miR-491-5p determined by Western blot analysis. vs. SW1990 cells transduced with mimic NC, inhibitor NC, PBS, or Vector+NC mimic. ^# vs. SW1990 cells transduced with miR-491-5p mimic+Vector or NEAT1+NC mimic. All data were presented as deviation. Comparisons of data between two groups were analyzed by unpaired -test, and comparison of data among multiple groups was tested by one-way analysis of variance, followed by Tukey’s post hoc test. Cell experiments were independently repeated three times.

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