Extracellular Vesicle-Loaded Oncogenic lncRNA NEAT1 from Adipose-Derived Mesenchymal Stem Cells Confers Gemcitabine Resistance in Pancreatic Cancer via miR-491-5p/Snail/SOCS3 Axis

Figure 7

Effects of ADSC-EVs carrying NEAT1 on PCa progression and gemcitabine resistance via miR-491-5p/Snail/SOCS3 axis. SW1990 cells were treated with EVs alone or combined with overexpressed SOCS3. (a) Expression of NEAT1 and miR-491-5p determined by RT-qPCR and protein levels of Snail and SOCS3 in SW1990 cells determined by Western blot analysis. (b) Clonogenic potential of SW1990 cells detected by colony formation assay. (c) Migration of SW1990 cells detected by Transwell assay. (d) Resistance of SW1990 cells to gemcitabine detected by MTS assay. (e) Tumorigenicity of SW1990 cells cocultured with EVs alone or combined with SOCS3 overexpression treatment in mice (). vs. SW1990 cells treated with PBS+Vector+Gem or PBS +Vector, or mice injected with SW1990 cells treated with PBS+Vector; ^# vs. SW1990 cells treated with EVs+Vector or EVs+Vector+Gem, or mice injected with SW1990 cells treated with EVs+Vector. All data were presented as deviation. Comparison of data among multiple groups was tested by one-way analysis of variance (ANOVA), followed by Tukey’s post hoc test. Data at different time points were analyzed by repeated measurement ANOVA, followed by Tukey’s post hoc test. Cell experiments were independently repeated three times.

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