Exosomal miR-141-3p from PDLSCs Alleviates High Glucose-Induced Senescence of PDLSCs by Activating the KEAP1-NRF2 Signaling Pathway

Figure 1

Characterization of human PDLSCs and PDLSC-Exos. (a–d) Isolation and passage of human PDLSCs. The cells exhibited a classic spindle-shaped morphology. Scale bar, 100 μm. (e) Alizarin red staining of calcified nodules indicated the capacity of PDLSCs to develop into osteoblasts. Scale bar, 100 μm. (f) Oil red O staining indicated the adipogenic differentiation capacity. Scale bar, 100 μm. (g) Clone formation assay showing the proliferation of human PDLSCs. Scale bar, 100 μm. (h) TEM observations revealed the typical cup-like shape of PDLSC-Exos. Scale bar, 100 nm. (i) WB showed that PDLSC-Exos expressed exosome marker proteins. (j) NTA revealed the particle size and concentration of PDLSC-Exos.

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