Exosomal miR-141-3p from PDLSCs Alleviates High Glucose-Induced Senescence of PDLSCs by Activating the KEAP1-NRF2 Signaling Pathway

Figure 2

High glucose-induced senescence in human PDLSCs. PDLSCs were treated with normal glucose (NG group; 5.5 mM glucose), high mannitol (HM group; 5.5 mM  mM mannitol), and high glucose (HG group; 25 mM glucose) to observe phenotypic changes in senescence. (a) After 14 days of high glucose administration, an increase in the proportion of SA-β-gal-positive cells (blue) was clearly visible after SA-β-gal labeling. Scale bar, 100 μm. (b) Quantification of the percentages of SA-β-gal-positive PDLSCs in the indicated groups. (c) Cell apoptosis was observed by Hoechst 33258 staining, and apoptotic nuclei were detected as condensed (white arrows). Scale bar, 100 μm. (d) Expression of p16 (green), as measured using IF staining. Scale bar, 100 μm. (e, f) WB and quantitative analyses of cellular senescence-related protein expression (p53 and p21). (g) The expression of the indicated SASP (IL-6 and IL-8) genes was quantified using qRT–PCR. All data are presented as the ( independent observations). NS indicates not significant, , , and . The corresponding control is indicated.

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