Exosomal miR-141-3p from PDLSCs Alleviates High Glucose-Induced Senescence of PDLSCs by Activating the KEAP1-NRF2 Signaling Pathway

Figure 5

PDLSC-Exos decrease oxidative stress and increase the activity of the endogenous NRF2 antioxidant system. (a, b) Intracellular ROS levels were evaluated by measuring DCFH-DA fluorescence. Scale bar, 100 μm. (c, d) The level of oxidative stress in cells was measured by detecting MDA levels and SOD activity. (e, f) Protein expression levels of total NRF2, nuclear NRF2, KEAP1, HO-1, and NQO1 in the indicated groups. (g) Localization of NRF2 (green), as visualized using IF staining. Scale bar, 100 μm. All data are presented as the ( independent observations). , , , and . The corresponding control is indicated.

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