Exosomal miR-141-3p from PDLSCs Alleviates High Glucose-Induced Senescence of PDLSCs by Activating the KEAP1-NRF2 Signaling Pathway

Figure 6

PDLSC-Exos rejuvenate senescent human PDLSCs by activating NRF2. High glucose-induced senescent human PDLSCs were treated with either PDLSC-Exos alone (HG-Exos group) or in combination with an NRF2 inhibitor (HG-Exos-ML385 group), while untreated senescent PDLSCs were used as controls (HG group). (a, b) WB analysis of total NRF2, nuclear NRF2, HO-1, and NQO1 levels in the indicated groups. (c) IF staining to detect the expression and localization of NRF2 (green). Scale bar, 100 μm. (d, e) Cells were stained with SA-β-gal, and activity was determined by counting the number of SA-β-gal-positive cells. Scale bar, 100 μm. (f, g) WB and quantification of senescence-related proteins (p53 and p21). (h) IF staining indicating p16 expression. Scale bar, 100 μm. (i) SASP gene (IL-6 and IL-8) expression levels were assessed using qRT–PCR. (j, k) Cellular ROS levels were detected and quantified by performing DCFH-DA staining. Scale bar, 100 μm. (l, m) MDA levels and SOD activity were determined. All data are presented as the ( independent observations). , , , and . The corresponding control is indicated.

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