Exosomal miR-141-3p from PDLSCs Alleviates High Glucose-Induced Senescence of PDLSCs by Activating the KEAP1-NRF2 Signaling Pathway

Figure 7

miR-141-3p transferred by PDLSC-Exos activates NRF2 signaling by reducing KEAP1 expression. (a) Venn diagram showing the miRNAs (hsa-miR-141-3p and hsa-miR-200a-3p) predicted to target the KEAP1 gene. (b) The expression levels of miRNAs in young and senescent PDLSCs were measured by qRT–PCR. (c) The expression levels of miRNAs in PDLSC-Exos were measured by qRT–PCR. (d) miR-141-3p expression was measured by qRT–PCR analysis. (e, f) Verification of the targeting relationship between miR-141-3p and KEAP1 using a dual-luciferase reporter gene assay. (g) qRT–PCR analysis was used to determine the expression levels of miR-141-3p in exosomes from treated human PDLSCs. NCI-Exos or 141I-Exos were used to treat high glucose-induced senescent human PDLSCs (HG-NCI-Exos group or HG-141I-Exos group), while untreated aged PDLSCs served as the control (HG group). (h, i) Total NRF2, nuclear NRF2, KEAP1, HO-1, and NQO1 protein levels were measured using WB and quantified using ImageJ software. (j) NRF2 expression levels were quantified, and NRF2 localization was identified by performing IF staining. Scale bar, 100 μm. All data are presented as the ( independent observations). NS indicates not significant, , , , and . The corresponding control is indicated.

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