Exosomal miR-141-3p from PDLSCs Alleviates High Glucose-Induced Senescence of PDLSCs by Activating the KEAP1-NRF2 Signaling Pathway

Figure 8

Exosomal miR-141-3p from PDLSCs is essential for preventing PDLSC senescence caused by high-glucose treatment. (a, b) Representative image of SA-β-gal staining along with the percentage of SA-β-gal-positive cells. Scale bar, 100 μm. (c, d) The expression levels of p53 and p21 were analyzed using WB. (e) Expression of p16 detected using IF staining. Scale bar, 100 μm. (f) Relative levels of the SASP genes IL-6 and IL-8. (g, h) Cellular ROS levels were detected and quantified by DCFH-DA staining. Scale bar, 100 μm. (i, j) MDA levels and SOD activity were assessed to evaluate the level of oxidative stress in cells. All data are presented as the ( independent observations). , , , and . The corresponding control is indicated.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)