Lama2 And Samsn1 Mediate the Effects of Brn4 on Hippocampal Neural Stem Cell Proliferation and Differentiation

Figure 4

Lama2 or Samsn1 knockdown promotes the proliferation of hippocampal NSCs. (a, b) Quantification of Lama2 and Samsn1 mRNA levels normalized to β-actin in the primary hippocampal NSCs which were infected with Lama2-shRNA lentivirus or Samsn1-shRNA lentivirus. (c, d) Representative immunoblots of Samsn1 and β-actin after being infected by Samsn1-shRNA lentivirus (c) and quantification of Samsn1 protein level normalized to β-actin (d). (e) Representative EdU-staining after Lama2 or Samsn1 knockdown in primary hippocampal NSCs. . (f) Quantification of EdU-positive cells normalized to Hoechst. (g–j) Representative cell cycle distribution analyzed by flow cytometry after Lama2 or Samsn1 knockdown in primary hippocampal NSCs (g–i) and quantification for NSCs in the different stages of the cell cycle (j). (k) CCK-8 assay in primary hippocampal NSCs 0, 1, 2, 3, and 4 days after lentivirus infection. Error bars represent the . . , , .

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