Research Article
Lama2 And Samsn1 Mediate the Effects of Brn4 on Hippocampal Neural Stem Cell Proliferation and Differentiation
Figure 6
Lama2 activation or Samsn1 overexpression promotes NSCs differentiation into neurons. (a, b) Representative immunostaining for Tuj1 (red) in the primary hippocampal NSCs after induced differentiation for 7 days following plasmid transfection or lentivirus infection. . (c, d) Quantification of Tuj1-positive cells normalized to Hoechst. (e–j) Representative immunoblots of Tuj1, GFAP, and β-actin in the primary hippocampal NSCs after induced differentiation for 7 days following plasmid transfection or lentivirus infection (e, f) and quantification of Tuj1 and GFAP protein levels normalized to β-actin (g–j). (k–n) Quantification of MAP2 and S100b mRNA levels normalized to β-actin after induced differentiation for 7 days following plasmid transfection or lentivirus infection. Error bars represent the . . , , .
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