Lama2 And Samsn1 Mediate the Effects of Brn4 on Hippocampal Neural Stem Cell Proliferation and Differentiation

Figure 7

Lama2 or Samsn1 knockdown inhibits NSCs differentiation into neurons. (a) Representative immunostaining for Tuj1 (red) in the primary hippocampal NSCs after induced differentiation for 7 days following lentivirus infection. . (b) Quantification of Tuj1-positive cells normalized to Hoechst. (c–e) Representative immunoblots of Tuj1, GFAP, and β-actin in the primary hippocampal NSCs after induced differentiation for 7 days following lentivirus infection (c) and quantification of Tuj1 and GFAP protein levels normalized to β-actin (d, e). (f, g) Quantification of MAP2 and S100b mRNA levels normalized to β-actin after the NSCs induced differentiation for 7 days following lentivirus infection. Error bars represent the . . , , .

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