SETD4 Confers Cancer Stem Cell Chemoresistance in Nonsmall Cell Lung Cancer Patients via the Epigenetic Regulation of Cellular Quiescence

Figure 4

SETD4 confers resistance to chemoradiotherapy by facilitating cell quiescence. (a) Protein levels of SETD4, Ki67, and PCNA in CD133-sorted LCSCs (derived from H1299 cell lines) overexpressing GFP (LCSCs^GFP) or SETD4 (LCSCs^SETD4). . GAPDH was used as an internal reference. . (b) Representative IF images of Ki67 in LCSCs^SETD4 and LCSCs^GFP. DAPI, nuclear counterstaining. Scale bar, 50 μm. Representative images and survival rate analysis of LCSCs^GFP and LCSCs^SETD4 after treatment with Taxol (TAX) (200 nmol/L)+CDDP (10 μmol/L)+radiation (c), pemetrexed (PEM) (200 nmol/L)+CDDP (10 μmol/L)+radiation (d), and gemcitabine (GEM) (0.5 μmol/L)+cisplatin (CDDP) (10 μmol/L)+radiation (e). . Scale bar, 50 μm. ; . The data are presented as the .

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