SETD4 Confers Cancer Stem Cell Chemoresistance in Nonsmall Cell Lung Cancer Patients via the Epigenetic Regulation of Cellular Quiescence

Figure 6

SETD4 controlled cell quiescence by inhibiting the PI3K-mTOR pathway via H4K20me3 catalysis. Representative IF images of H4K20me3 (a) and HP1-α (b) in qLCSCs and A-qLCSCs derived from H1299 and H520 cell lines. DAPI, nuclear counterstaining. Scale bar, 50 μm. (c) Protein levels of H4K20me3 and HP1-α in qLCSCs and A-qLCSCs. H4 was used as an internal reference. . . Representative IF images of H4K20me3 (d) and HP1-α (e) in A-qLCSCs^SETD4 and A-qLCSCs^GFP. DAPI, nuclear counterstaining. Scale bar, 50 μm. (f) Protein levels of H4K20me3 and HP1-α in A-qLCSCs^SETD4 and A-qLCSCs^GFP. H4 was used as an internal reference. . (g) Protein levels of PTEN, mTOR, PI3K, and p-PI3K in A-qLCSCs^SETD4 and A-qLCSCs^GFP. GAPDH was used as an internal reference. GAPDH was used as an internal reference. . Data are presented as the .

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