Platelet-Derived Mitochondria Attenuate 5-FU-Induced Injury to Bone-Associated Mesenchymal Stem Cells

Figure 1

5-FU induces BA-MSCs injury in vitro and in vivo. (a) Schematic of BA-MSC injury model induced by 5-FU in vitro. (b) Flow cytometric plots of apoptosis detection of BA-MSCs injured by 5-FU. (c) Flow cytometry analysis of annexin V⁺ BA-MSC frequency in 5-FU-induced BA-MSC injury model in vitro (). (d) Analysis of total cell number of BA-MSCs in 5-FU-induced BA-MSC injury model in vitro (). (e) Flow cytometric plots of cell cycle and BA-MSC cell cycle statistical analysis in 5-FU-induced BA-MSC injury model in vitro (). (f) Schematic of mouse myelosuppression model induced by 5-FU in vivo. (g) Flow cytometric plots of BMC subsets (HSCs and HPCs). (h) Statistical analysis of percentage of HSPCs in BMCs in myelosuppression mouse model (). (i) Analysis of total cell number of BMCs in myelosuppression mouse model (). (j) Flow cytometric plots of apoptosis detection of BA-MSCs in myelosuppression mouse model. (k) Flow cytometry analysis of annexin V⁺ BA-MSC frequency in myelosuppression mouse model (). (l) Analysis of total cell number of BA-MSCs in myelosuppression mouse model (). (m) Flow cytometric plots of cell cycle and BA-MSC cell cycle statistical analysis in myelosuppression mouse model (). (a–e) BA-MSCs in 5-FU group were treated with 12.5 μM 5-FU for 48 h; BA-MSCs in control group were treated with 12.5 μM DMSO for 48 h. (f–m) Mice of 5-FU group were intraperitoneally injected with 5-FU (150 mg/kg, 200 μL) for 6 days; mice of control group were intraperitoneally injected with PBS (200 μL) for 6 days. All data are presented as . Statistics: unpaired Student’s -test (ns: not significant; , , , and ).

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